Anaerobic susceptibility tests. Evaluation of the stability of antimicrobials in Wilkens-Chalgren broth and the effect of media prereduction.
نویسندگان
چکیده
The stability of eight antimicrobial agents in WILKENS-CHALGREN broth was evaluated. The activities of only carbenicillin and benzyl penicillin were significantly decreased after storage for eight weeks at--20 degrees C. Anaerobic reduction of the susceptibility testing broth prior to inoculation was found to be unnecessary.
منابع مشابه
Growth of Peptococcus and Peptostreptococcus: effect of variations of culture media on efficiency of recovery.
Reference strains and clinical isolates of Peptococcus and Peptostreptococcus spp. were evaluated for their growth response in supplemented thioglycolate-yeast extract media. Supplements used included various combinations of hemin, menadione, sodium bicarbonate, and Tween 80. Parallel studies were done to compare the efficiency of recovery of viable cells grown in thioglycolate-based media and ...
متن کاملEvaluation of biofilm-forming capabilities of urinary Escherichia coli isolates in microtiter plate using two different culture media
The ability of uropathogenic E. coli (UPEC) to cause symptomatic UTI is associated with the expression of a variety of virulence factors. Biofilm formation enables UPEC to resist the flow of urine and increases its tolerance to antimicrobials and the host immune response. The measurement of biofilm formation in vitro is affected by the type of culture medium used. The aim of this study was to e...
متن کاملVaspar broth-disk procedure for antibiotic susceptibility testing of anaerobic bacteria.
A modification of the Wilkins-Thiel broth-disk procedure for antibiotic susceptibility testing of anaerobic bacteria is described. This method utilizes an aerobically prepared medium overlaid with molten vaspar. Specialized anaerobic techniques or prereduced media are not required.
متن کاملEffect of culture medium and carbon dioxide concentration on growth of anaerobic bacteria commonly encountered in clinical specimens.
Representative strains of anaerobic bacteria from human infections were used to evaluate broth media, gas mixtures, and inocula for use in developing a procedure for performing minimal inhibitory concentration antimicrobic susceptibility tests. Nine commercially available media, including two that were chemically defined, were tested. Tests were performed in atmospheres with carbon dioxide conc...
متن کاملValidation of Integral System Yeast Plus for rapid identification and determination of antifungal susceptibility profile of clinically important Candida species
Precise identification of microorganisms involved in candidiasis together with antifungal susceptibility evaluation could help clinicians to prescribe appropriate medicine, especially in patients with critical conditions. The present study has been conducted to evaluate discriminatory power of Integral System Yeast Plus (ISYP) for rapid identification and determination of antifungal susceptibil...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The Journal of antibiotics
دوره 31 12 شماره
صفحات -
تاریخ انتشار 1978